cDNA Cloning and Characterization of Human Monocyte/Macrophage Serine Esterase-1
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چکیده
Human monocyte/macrophage serine esterase (HMSE), commonly known as acid esterase or anaphthylacetate esterase, comprises a group of five enzyme variants that can be distinguished by their isoelectric points from esterase variants of the other normal human blood cell populations. A cDNA for one of the monocytic enzyme variants (HMSE1) was cloned from a U-937 A g t l l cDNA library by screening with an oligonucleotide mixture designed according to amino acid sequence data of the purified enzyme. The cDNA contains 1,727 bp with an open reading frame of 1,512 bp coding for a protein of 503 amino acid residues. HMSEl cDNA
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cDNA Cloning and Characterization of Human Monocyte/Macrophage Serine Esterase-1
Human monocyte/macrophage serine esterase (HMSE), commonly known as acid esterase or anaphthylacetate esterase, comprises a group of five enzyme variants that can be distinguished by their isoelectric points from esterase variants of the other normal human blood cell populations. A cDNA for one of the monocytic enzyme variants (HMSE1) was cloned from a U-937 A g t l l cDNA library by screening ...
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